Sequence alignment of N-terminal ends of peptides encoded by the IL4I1 gene (Il4i1) and the novel variant (Il4i1_2). Sequences encoded by the first coding exon are different between the two variants, and the splice site is indicated with a red arrowhead. The mouse sequence of Il4I1_2 was derived from the following ESTs: BY100275, BY099330, BY087056, BY092834 and BY088421. The rat sequence was translated from the EST CV117152. Dog and chimpanzee sequences were deduced from aligned genomic sequences . The cleavage site of the signal peptidase is predicted at the same position in all sequences and is indicated with a blue arrow head.