-/- islets remain in ductal proximity and have a streaked appearance at E18.5. (A) In Wnt5a
-/- embryos, most islets are associated with ducts. Both small and large β-cell aggregates are more frequently associated with pancreatic ducts in Wnt5a
-/- embryos at E18.5 than in wild-type embryos. (B, C) Insulin antibody staining. (B) Round and compact islets in wild-type embryos. A normal pancreas consists of islets that are associated and separated from ducts. Arrows: pancreatic duct, asterisk: an islet separated from duct. (C) Streak-like, fragmented islets in Wnt5a
-/- mutant embryos.