Figure 1

Drosophila CG2095 / sec8. A: Amino acid alignment of human (Accession number NP_068579), rat (Accession number Q62824), fly (Accession number AY905551), nematode (Accession number NP_492732) and yeast (Accession number NP_015380) Sec8. The amino acid sequence of Drosophila CG2095 is 33% identical to rat and human Sec8. Drosophila amino acids 440–460 (underlined) represent the peptide sequence used to generate Sec8 antibodies. B: Genomic map of Drosophila CG2095 (sec8). Grey bars represent exons. sec8P1 is a lethal transposon insertion into the first exon. sec8Δ1 is a deletion created by imprecise excision of the transposon. The sec8Δ1 deletion removes almost the entire sec8 gene, with the exception of the first 37 bp of exon 1 and last 73 bp of the final exon 9.