Figure 2

Sec8 is found in the Drosophila NMJ. A: Third instar larval NMJs on ventral longitudinal muscles 6 and 7, stained using antibodies against Sec8 (green), GluRIIA (red), and the neuronal membrane marker anti-HRP (blue). All results in this study are derived from experiments on 6/7 NMJs in segments A3–A4. Lower row shows NMJ terminals at higher magnification. Scale bars = 5 μm. B: Confocal fluorescent images showing intersegmental nerve branch b (ISNb) innervating ventral longitudinal muscles in first instar larvae (24–28 h AEL). NMJs were visualized using the neuronal membrane marker anti-HRP (magenta). Sec8 was visualized using anti-Sec8 antibodies (green). Scale bar: 10 μm. Insets show individual synaptic boutons at higher magnification (approximately 2 μm × 2 μm). C: Quantification of total animal Sec8 protein, measured using protein blots from first instar control and mutant larvae, probed with anti-Sec8 antibody.