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Figure 3 | BMC Biology

Figure 3

From: Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling

Figure 3

Agonist-induced internalization is reduced in Rog-A, but not Rog-μ. (A) Confocal micrographs showing localization of GFP-tagged Rog (left), Rog-A (center), or Rog-μ (right) stably expressed in HEK293 cells and treated with 10 μM spiradoline for 1 h before fixation. (B) A dose-response ELISA shows less internalization of Rog-A in response to 1 h of spiradoline at doses of 0.3–100 μM spiradoline. After treatment with 1 μM spiradoline, Rog-A showed less internalization up to 1 h after treatment. (C) A dose-response ELISA shows more internalization of Rog-μ in response to 1 h of low doses of spiradoline ranging from 0.01 to 0.1 μM. After treatment with 1 μM spiradoline, there was no difference in the time course of internalization between Rog and Rog-μ. ELISA data are expressed as a percentage of receptors detected on surface of untreated cells expressing the same receptor.

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