Figure 4

Antagonist treatment increases cell-surface expression of Rog-μA. (A) ELISA comparing cell-surface expression of receptors stably expressed in HEK293 cell lines, either untreated (white bars) or treated for 18 h with the antagonist norBNI (10 μM, dark bars). Error bars represent SEM for three replicates. NorBNI significantly increased cell-surface expression of both Rog-μ and Rog-μA. OD, optical density. (B) Confocal micrographs showing that the localization of GFP-tagged Rog-μA is primarily intracellular in untreated cells (left), but the receptor moves to the membrane after 18 hours of antagonist treatment (right). There is relatively little change in Rog after norBNI treatment relative to untreated cells.