Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling

Table 1 EC50 and Emax values for inhibition of cAMP accumulation by spiradoline

	Rog	Rog-A	Rog-μ	Rog-μA
	EC50 (nM spiradoline)
Acute activation	0.41 ± 0.08	0.41 ± 0.15	0.42 ± 0.23	0.50 ± 0.22
1 nM spiradoline pretreatment	4.32 ± 1.96	0.42 ± 0.21	2.05 ± 0.03	> 13.44 ± 5.41
	% Inhibition
Acute activation	64.4 ± 4.8	56.1 ± 5.1	58.0 ± 0.9	64.1 ± 4.8
1 nM spiradoline pretreatment	55.7 ± 3.7	53.3 ± 0.9	43.6 ± 1.5	51.5 ± 6.0

ISSN: 1741-7007