Figure 1

Maps of the constructs used for homologous recombination. Central map shows the structure of the linearized pSSU vector. 5'SSU and 3'SSU correspond to the sequences required for homologous recombination into an 18s rRNA gene; SL refers to a sequence containing a splice acceptor site; MCS to the multiple cloning site; pac to the puromycin gene and CPB-2.8 IR to the intergenic region of the CPB 2.8 gene of L. mexicana. The top map shows the structure of alt-1 and alt-2 transcripts that were introduced into the MCS of pSSU. The bottom map shows putative intergration into a rRNA locus. The arrow shows the start of transcription from the rRNA genes.