Figure 5
H 2 O 2 induces PIP 3 production in an age-1 -dependent manner. Starved L1 larvae were 32P-orthophosphate labelled and the phosphoinositides were purified, deacylated and resolved using thin layer chromatography with a PIP3 standard. Autoradiographs show that (A) H2O2 induces PIP3 in wild type (N2) but not age-1(mg44); daf-16(m27) larvae. (B) Increasing concentrations of H2O2 causes increasing PIP3 in wild type (N2) larvae but no PIP3 is detectable in age-1(hx546) larvae.