Table 1 Translocation of DAF-16 to cytoplasm in ageing worms. Adult worms containing the muIs71 DAF-16a::GFP transgene and either wild type or the hx546 mutant allele at the age-1 locus were kept in isolated cohorts at 20°C and scored for DAF-16::GFP localisation using a fluorescent compound microscope. Nuclei were scored as either distinctly visible or not distinguishable from the cytoplasm. See Figure 6 for images.
age-1 allele | Â | No. of worms | |||||
|---|---|---|---|---|---|---|---|
| Â | DAF-16 localisation | DAY 2 | DAY 4/5 | DAY 6/7 | DAY 8/9 | DAY 11 | DAY 14 |
wild type | distinct nuclei | 28 | 13 | 5 | 1 | Â | Â |
| Â | nuclei not visible | 0 | 12 | 8 | 16 | Â | Â |
age-1(hx546) | distinct nuclei | 18 | 19 | 6 | 29 | 14 | 13 |
| Â | nuclei not visible | 0 | 0 | 0 | 0 | 0 | 0 |