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Table 3 BIAcore analysis of IL-4Rα variants

From: A modular interface of IL-4 allows for scalable affinity without affecting specificity for the IL-4 receptor

  IL-4 IL4-T13D
IL4-Rα variant k on × 10-7 [s-1 M-1] k off × 103 [s-1] app. K D [nM] k on × 10-7 [s-1 M-1] k off × 103 [s-1] app. K D [nM]
IL4-Rα 1.79 ± 0.27 1.62 ± 0.67 0.09 ± 0.04 1.24 ± 0.20 0.25 ± 0.20 0.02 ± 0.02
Y13T 1.55 ± 0.67 23.5 ± 4.05 1.76 ± 0.75 1.03 ± 0.43 5.38 ± 0.51 0.66 ± 0.43
D67A 0.80 ± 0.34 36.5 ± 7.60 5.60 ± 3.32 0.52 ± 0.19 1.82 ± 0.39 0.38 ± 0.10
D125A 0.89 ± 0.31 6.46 ± 1.75 0.77 ± 0.23 0.79 ± 0.14 0.65 ± 0.29 0.08 ± 0.04
Y127F 1.17 ± 0.51 1.99 ± 0.65 0.20 ± 0.07 0.96 ± 0.15 0.91 ± 0.34 0.09 ± 0.02
  IL4-F82D IL4-T13D-F82D
IL4-Rα variant k on × 10-7 [s-1 M-1] k off × 103 [s-1] app. K D [nM] k on × 10-7 [s-1 M-1] k off × 103 [s-1] app. K D [nM]
IL4-Rα 1.56 ± 0.93 0.48 ± 0.37 0.04 ± 0.04 1.06 ± 0.26 1.44 ± 0.96 0.14 ± 0.11
Y13T 1.20 ± 0.36 8.64 ± 1.74 0.76 ± 0.21 0.68 ± 0.32 31.8 ± 3.42 4.22 ± 0.62
D67A 0.68 ± 0.18 8.82 ± 1.83 1.37 ± 0.47 0.52 ± 0.11 8.56 ± 1.63 1.67 ± 0.27
D125A 0.99 ± 0.22 2.02 ± 0.39 0.21 ± 0.06 0.55 ± 0.11 1.34 ± 0.40 0.24 ± 0.06
Y127F 1.12 ± 0.21 1.08 ± 0.33 0.10 ± 0.03 0.40 ± 0.24 27.4 ± 6.01 6.15 ± 2.68
  1. Association and dissociation rates of IL-4 variants to immobilized IL4-RαECD variants were measured on a BIA2000 system. The rate constants represent mean values of 12 independent measurements with 6 different analyte concentrations.