| IL-4 | IL4-T13D | ||||
---|---|---|---|---|---|---|
IL4-Rα variant | k on × 10-7 [s-1 M-1] | k off × 103 [s-1] | app. K D [nM] | k on × 10-7 [s-1 M-1] | k off × 103 [s-1] | app. K D [nM] |
IL4-Rα | 1.79 ± 0.27 | 1.62 ± 0.67 | 0.09 ± 0.04 | 1.24 ± 0.20 | 0.25 ± 0.20 | 0.02 ± 0.02 |
Y13T | 1.55 ± 0.67 | 23.5 ± 4.05 | 1.76 ± 0.75 | 1.03 ± 0.43 | 5.38 ± 0.51 | 0.66 ± 0.43 |
D67A | 0.80 ± 0.34 | 36.5 ± 7.60 | 5.60 ± 3.32 | 0.52 ± 0.19 | 1.82 ± 0.39 | 0.38 ± 0.10 |
D125A | 0.89 ± 0.31 | 6.46 ± 1.75 | 0.77 ± 0.23 | 0.79 ± 0.14 | 0.65 ± 0.29 | 0.08 ± 0.04 |
Y127F | 1.17 ± 0.51 | 1.99 ± 0.65 | 0.20 ± 0.07 | 0.96 ± 0.15 | 0.91 ± 0.34 | 0.09 ± 0.02 |
 | IL4-F82D | IL4-T13D-F82D | ||||
IL4-Rα variant | k on × 10-7 [s-1 M-1] | k off × 103 [s-1] | app. K D [nM] | k on × 10-7 [s-1 M-1] | k off × 103 [s-1] | app. K D [nM] |
IL4-Rα | 1.56 ± 0.93 | 0.48 ± 0.37 | 0.04 ± 0.04 | 1.06 ± 0.26 | 1.44 ± 0.96 | 0.14 ± 0.11 |
Y13T | 1.20 ± 0.36 | 8.64 ± 1.74 | 0.76 ± 0.21 | 0.68 ± 0.32 | 31.8 ± 3.42 | 4.22 ± 0.62 |
D67A | 0.68 ± 0.18 | 8.82 ± 1.83 | 1.37 ± 0.47 | 0.52 ± 0.11 | 8.56 ± 1.63 | 1.67 ± 0.27 |
D125A | 0.99 ± 0.22 | 2.02 ± 0.39 | 0.21 ± 0.06 | 0.55 ± 0.11 | 1.34 ± 0.40 | 0.24 ± 0.06 |
Y127F | 1.12 ± 0.21 | 1.08 ± 0.33 | 0.10 ± 0.03 | 0.40 ± 0.24 | 27.4 ± 6.01 | 6.15 ± 2.68 |