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Table 3 BIAcore analysis of IL-4Rα variants

From: A modular interface of IL-4 allows for scalable affinity without affecting specificity for the IL-4 receptor

 

IL-4

IL4-T13D

IL4-Rα variant

k on × 10-7 [s-1 M-1]

k off × 103 [s-1]

app. K D [nM]

k on × 10-7 [s-1 M-1]

k off × 103 [s-1]

app. K D [nM]

IL4-Rα

1.79 ± 0.27

1.62 ± 0.67

0.09 ± 0.04

1.24 ± 0.20

0.25 ± 0.20

0.02 ± 0.02

Y13T

1.55 ± 0.67

23.5 ± 4.05

1.76 ± 0.75

1.03 ± 0.43

5.38 ± 0.51

0.66 ± 0.43

D67A

0.80 ± 0.34

36.5 ± 7.60

5.60 ± 3.32

0.52 ± 0.19

1.82 ± 0.39

0.38 ± 0.10

D125A

0.89 ± 0.31

6.46 ± 1.75

0.77 ± 0.23

0.79 ± 0.14

0.65 ± 0.29

0.08 ± 0.04

Y127F

1.17 ± 0.51

1.99 ± 0.65

0.20 ± 0.07

0.96 ± 0.15

0.91 ± 0.34

0.09 ± 0.02

 

IL4-F82D

IL4-T13D-F82D

IL4-Rα variant

k on × 10-7 [s-1 M-1]

k off × 103 [s-1]

app. K D [nM]

k on × 10-7 [s-1 M-1]

k off × 103 [s-1]

app. K D [nM]

IL4-Rα

1.56 ± 0.93

0.48 ± 0.37

0.04 ± 0.04

1.06 ± 0.26

1.44 ± 0.96

0.14 ± 0.11

Y13T

1.20 ± 0.36

8.64 ± 1.74

0.76 ± 0.21

0.68 ± 0.32

31.8 ± 3.42

4.22 ± 0.62

D67A

0.68 ± 0.18

8.82 ± 1.83

1.37 ± 0.47

0.52 ± 0.11

8.56 ± 1.63

1.67 ± 0.27

D125A

0.99 ± 0.22

2.02 ± 0.39

0.21 ± 0.06

0.55 ± 0.11

1.34 ± 0.40

0.24 ± 0.06

Y127F

1.12 ± 0.21

1.08 ± 0.33

0.10 ± 0.03

0.40 ± 0.24

27.4 ± 6.01

6.15 ± 2.68

  1. Association and dissociation rates of IL-4 variants to immobilized IL4-RαECD variants were measured on a BIA2000 system. The rate constants represent mean values of 12 independent measurements with 6 different analyte concentrations.