Table 4 MAD data set for IL-4 variant F82D
Crystal | F82D | ||
|---|---|---|---|
Space group Cell constants | P41212 a = b = 91.304 Å, c = 45.932 Å α = β = γ = 90° | ||
|  | Se-Met (λ1) | Se-Met (λ2) | Se-Met (λ3) |
Wavelength | 0.9844 Ã… | 0.9803 Ã… | 0.9078 Ã… |
Resolution (Å)1 | 25.0 – 2.15 Å (2.27 – 2.15 Å) | 25.0 – 2.15 Å (2.27 – 2.15 Å) | 25.0 – 2.15 Å (2.27 – 2.15 Å) |
|  | λ1 (inflection) | λ2 (peak) | λ3 (remote high) |
Number of measured reflections | 51335 (5658) | 54273 (5689) | 50834 (5624) |
Number of unique reflections | 10758 (1410) | 10721 (1413) | 10640 (1384) |
Completeness | 97.0 (90.6) | 97.3 (91.2) | 96.5 (89.2) |
Multiplicity | 4.8 (4.0) | 5.1 (4.0) | 4.8 (4.1) |
Rsym for all reflections2 | 5.6 (13.8) % | 6.0 (12.1) % | 4.7 (10.3) % |
Intensity/σ | 18.1 (7.4) | 18.2 (8.0) | 20.1 (11.0) |
Phasing | Â | Â | Â |
Rcullis (a/c)3 | 0.689/0.533 | 0.725/0.651 | - |
RKraut 4 | 0.035 | 0.034 | 0.028 |
Phasing Power (a/c)5 | 1.60/1.90 | 1.42/1.65 | 0.93/1.09 |
Figure of merit6 | 0.55/0.79 (after DM) | Â | Â |