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Table 1 Degenerate primers designed and used in this study

From: Three rounds (1R/2R/3R) of genome duplications and the evolution of the glycolytic pathway in vertebrates

Name Sequence Enzyme
Eno.uni.FN1 GGN AAY CCI ACI GTN GAR GT Enolase
Eno.uni.FN2 ACI GGI ATH TAY GAR GC Enolase
Eno.uni.FM AAR TAY GGI AAR GAY GC Enolase
Eno.uni.RC1 GT RTC YTC IGT YTC NCC Enolase
Eno.uni.RC2 GC ICC IGT YTT DAT YTG Enolase
Eno.uni210.F ACC AAC GTT GGI GAY GAR GGI GG Enolase
Eno.uni320.R TTG GTC ACA GTI ARR TCT TCI CC Enolase
FBA.Super+ GGT AAR GGT ATC YTI GCI GCI GAY G Fructose-Bisphosphate Aldolase
FBA.uni.125+ ACT ACT AYT CAG GGN YTN GAY GG Fructose-Bisphosphate Aldolase
FBA.uni.240- GC ATG ACC AGS AGT NAC CAT RTT NGG Fructose-Bisphosphate Aldolase
FBA.uni.315- CAG AGC AGW AGC CTG CAG NGC NCK NCC Fructose-Bisphosphate Aldolase