Innervation of the ovary by a single type of aminergic fibre. A. Merged confocal images from a Wee-P26 fly of nerves immunostained with anti-HRP (magenta) and of muscle expressing MHC-GFP (green) show that the nerves run along the muscle fibres of the peritoneal sheath with varicosities at irregular intervals and at their ends. The thinner muscle fibres running vertically are part of the epithelial sheath around an ovariole. B. A similar image showing a single neural varicosity at higher magnification. C. Merged confocal images showing nerves (magenta) immunostained as above in a fly expressing CD8-GFP-Shaker (green). The absence of detectable green fluorescence indicates a lack of Type I neuromuscular junctions. D. Individual and merged confocal images showing that the expression of dTdc2-GAL4 as reported by UAS-n-syb-spH (green) co-localising (white) with nerve fibres immunostained as above (magenta). E. Individual and merged confocal images of a specimen double-labelled with anti-HRP (magenta) and an antibody to the Drosophila vesicular monoamine transporter (DVMAT, green), shows that the DVMAT staining is co-incident with neural varicosities and nerve endings with occasional smaller hot-spots along the length of the nerves. No DVMAT signal was detected in the muscle fibres. Scalebars: All 20 μm.