Figure 3

Hydrodynamic analysis of Myo1 MLD A. Superose 6 gel filtration calibration curve with positions of protein standards of known Stokes radii shown. The dotted lines show the position of the Myo1 MLD with a measured Stokes radius of 8.4 nm. B. Sucrose density gradient calibration curve with the positions of protein standards of known sedimentation coefficients shown. The dotted lines show the position of Myo1 MLD with a measured sedimentation coefficient of 5.2 S. C. Coomassie Blue stained SDS-PAGE gel showing Superose 6 FPLC fractions of bacterially expressed MLD (after GST tag removal). Fractions were TCA precipitated before loading on the gel. Numbers to the left of the gel show molecular weight of markers (M). D. Western blot of Superose 6 FPLC fractions of MLD-(myc)6 yeast extract using anti-myc antibodies. Fractions were TCA precipitated before loading on the gel. Horizontal black marks and numbers to the left of the marks show molecular weight of markers (M).