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Table 2 Strains

From: Characterization of the minimum domain required for targeting budding yeast myosin II to the site of cell division

Name   Genotype Background Source
RLY261   MATa ura3-1 his3-11,15 leu2-3,112 trp1 ade2-1 bar1Δ W303a Elion Lab
RLY621   MATa/α ura3/ura3 his3/his3 leu2/leu2 trp1/trp1 myo1Δ::TRP1/MYO1 S288c Li Lab
RLY622   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 S288c Li Lab
RLY751   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1928)-GFP:LEU2 (pNT13) W303a This work
RLY1034   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1569)-GFP-6myc:LEU2 (pNT49) W303a This work
RLY1035   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1-GFP-6myc:LEU2 (pNT50) W303a This work
RLY1040   MATa/α ura3-1/ura3-1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ade2-1/ade2-1 bar1Δ/bar1Δ myo1Δ::TRP/MYO1 W303a This work
RLY1044   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1-GFP-6myc:LEU2 (pNT50) S288c This work
RLY1046   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1044–1569)-GFP-6myc:LEU2 (pNT49) S288c This work
RLY1051   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1 Δ pMYO1(1–1043)-GFP-6myc:LEU2 (pNT51) W303a This work
RLY1073   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1253)-GFP-6myc:LEU2 (pNT62) W303a This work
RLY1074   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1261–1569)-GFP-6myc:LEU2 (pNT63) W303a This work
RLY1096   MATa/α ura3-1/ura3-1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ade2-1/ade2-1 bar1Δ/bar1Δ myo1Δ::TRP/MYO1 pMYO1(1-1902)-GFP-6myc:LEU2 (pNT66) W303a This work
RLY1101   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1–1902)-GFP-6myc:LEU2 (pNT66) W303a This work
RLY1103   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ myo1Δ::TRP pMYO1(1–1902)-GFP-6myc:LEU2 (pNT66) W303a This work
RLY1119   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1161)-GFP-6myc:LEU2 (pNT71) W303a This work
RLY1212   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1044–1928)-GFP-6myc:LEU2 (pNT76) S288c This work
RLY1288   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1161–1928)-GFP-6myc:LEU2 (pNT94) W303a This work
RLY1295   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(864–1161)-GFP-6myc:LEU2 (pNT96) W303a This work
RLY1295   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(864–1161)-GFP-6myc:LEU2 (pNT96) W303a This work
RLY1297   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1529–1928)-GFP:LEU2 (pNT40) W303a This work
RLY1298   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1529–1928)-GFP:LEU2 (pNT40) S288c This work
RLY1307   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1430)-GFP-6myc:LEU2 (pNT110) W303a This work
RLY1308   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1387)-GFP-6myc:LEU2 (pNT111) W303a This work
RLY1309   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1314)-GFP-6myc:LEU2 (pNT112) W303a This work
RLY1310   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1044–1430)-GFP-6myc:LEU2 (pNT110) S288c This work
RLY1311   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1044–1387)-GFP-6myc:LEU2 (pNT111) S288c This work
RLY1312   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1044–1314)-GFP-6myc:LEU2 (pNT112 S288c This work
RLY1330   MATa/α ura3-1/ura3-1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ade2-1/ade2-1 bar1Δ myo1Δ::KAN/MYO1 pMYO1-GFP-6myc:LEU2 (pNT113) W303a This work
RLY1331   MA/α ura3-1 his3-11 leu2-3,112 trp1-1 ade2-1 bar1Δ myo1Δ::KAN pMYO1-GFP-6myc:LEU2 (pNT113) W303a This work
RLY1577   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1044–1160:1431–1928)-GFP-6myc:LEU2 (pNT121) W303a This work
RLY1578   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1044–1160:1431–1928)-GFP-6myc:LEU2 (pNT121) S288c This work
RLY1591   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1 pMYO1(1161–1928)-GFP-6myc:LEU2 (pNT94) S288c This work
RLY1598   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ myo1Δ::TRP pMYO1-GFP-6myc:URA3 (pNT113) W303a This work
RLY1653   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ myo1Δ::TRP pMYO1-GFP-6myc:URA3 (pNT113) p:LEU2 (pRL304) W303a This work
RLY1655   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ myo1Δ::TRP pMYO1-GFP-6myc:URA3 (pNT113) g pMYO1-GFP-6myc:LEU2 (pNT50) W303a This work
RLY1657   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ myo1Δ::KAN pMYO1-GFP-6myc:URA3 (pNT113) pMYO1(1–1045:1570–1928)-GFP-6myc:LEU2 (pNT120) W303a This work
RLY1659   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1–1045:1570–1928)-GFP-6myc:LEU2 (pNT120) W303a This work
RLY1660   MATα ura3 his3 leu2 trp1 myo1Δ::TRP1g pMYO1(1–1045:1570–1928)-GFP-6myc:LEU2 (pNT120) S288c This work
RLY2127   MATα ura3 his3 leu2 trp1 myo1Δ::TRP, pMYO1(1–1160:1431–1928)-GFP-6myc:LEU (pIL11) S288c This work
RLY2128   MATa ura3-1 his3-11,15 leu2-3,112 trp1-1 ade2-1 bar1Δ pMYO1(1–1160:1431–1928-GFP-6myc):LEU (pIL11) W303a This work
RLY2132   MATα ura3 his3 leu2 trp1 MLD-6myc:LEU (pIL13) S288c This work
RLY2139   MATa ura3 his3 leu2 trp1myo1Δ::TRP1 pMYO1(1–1160:1431–1928) -GFP-6myc:LEU (pIL11) S288c This work