Figure 8
acr-5 promoter-driven expression in the male ventral cord motor neurons. (A–C) acr-5::GFP expression in wild-type males in the ventral cord of the posterior mid-body region. Ventral view with anterior oriented left. Nomarski (A), fluorescence (B) and merged (C) images. Animal shown is a pha-1(e2123ts) young adult male transformed with the extrachromosomal array pha-1+ acr-5::GFP. (D–F) tph-1::GFP (green) and acr-5::DsRed2 (red) expression in the male ventral cord. Nomarski (D), merged tph-1::GFP and acr-5::DsRed2 fluorescence (E), and merged (F) fluorescence and Nomarski images. Lateral views with posterior oriented left, and dorsal oriented upwards. Animal shown is a pha-1(e2123ts) young adult male transformed with the extrachromosomal array pha-1+ acr-5::DsRed2 and carrying the extrachromosomal array tph-1::GFP that was crossed into the strain. (G–I) acr-5::GFP in lin-39 (n1880) mutant males in the ventral cord of the posterior mid-body region. Ventral view with anterior oriented left. Nomarski (G), fluorescence (H), and merged (I) images. Animal shown is a young lin-39(n1880) mutant adult male that carries the same transgene used in (A–C) because it was crossed into the strain.