Figure 2
gna-2(qa705) is deficient in UDP-GlcNAc and its products. (A) gna-2(qa705) has a 1434-bp deletion that removes the entire coding sequence of gna-2 (T23G11.2.2). (B) HPLC traces (A254 nm) showing UDP-GlcNAc (red arrow), UDP-GalNAc (blue arrow) and UDP-Glc + UDP-Gal (asterisk) in gna-2(qa705) or wild type. (C) Hatchling number of (1) wild type, (2) sup-46(qa707), (3) sup-46(qa708), (4) gna-2(qa705)sup-46(qa707), (5) gna-2(qa705)sup-46(qa708) or (6) gna-2(qa705). Genotypes with different letters are significantly different (p < 0.05, n = 9, mean ± SEM). (D) Hatchling number of (I) wild type, (II) gna-1(RNAi), (III) gna-2(qa705)sup-46(qa707) or (IV) gna-2(qa705)sup-46(qa707); gna-1(RNAi). Genotypes with different letters are significantly different (p < 0.05, n = 10, bars are mean ± SEM). (E) Hatchling number of gna-2(qa705) following injection of UDP-GlcNAc into one distal gonad arm. Each point represents hatchlings of one injected hermaphrodite. (F) Wild type or gna-2(qa705) stained for DNA (Hoechst) and chitin or (G) DNA (Hoechst) and epitopes for the following lectins: WGA, LEA, STA, SBA. (F,G) Embryos are at the one-cell stage. Wild-type embryos are oriented with anterior (maternal DNA, polar-body end) on the left. gna-2(qa705) does not extrude polar bodies but where maternal meiotic DNA was visible, it was oriented on the left.