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Table 1 Summary of meiotic phenotype in gna-2(qa705), chs-1(RNAi) or cej-1+B0280.5(coRNAi) a

From: The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegansembryo

  controlb gna-2(qa705) c chs-1(RNAi) d cej-1+B0280.5(coRNAi) e
1 st polar body not extruded, 12 sister chromatids at meiosis II 0 100 100 76
2 nd polar body not extuded 0 100 100 82
stray chromatin at meiosis I f 0 8 0 6
stray chromatin at meiosis II f 0 15 10 12
SPCC failed to contact cortex g 0 69 30 47
no pseudocleavage furrow 0 100 100 94
  1. Numbers are % of embryos displaying phenotype
  2. aembryos examined in utero using a Leica DMLSFA Confocal Microscope.
  3. bwild type expressing histone H2B::GFP; n = 10
  4. c gna-2(qa705) expressing histone H2B::GFP; n = 13
  5. d chs-1(RNAi) expressing histone H2B::GFP; n = 10
  6. e cej-1+B0280.5(coRNAi) expressing histone H2B::GFP; n = 17
  7. f3rd, smaller mass of chromatin, separate from 2 major masses of chromatin segregated at anaphase
  8. gsperm pronucleus failed to come within 1/2 sperm pronucleus diameter of the cortex