|
Cross
|
Parents
| |
Progeny
| | | | | | | | | |
|---|
| |
Male
|
Female
|
Tc
|
Genotype
|
Egg
|
L1
|
L2
|
L3
|
L4
|
Pupae
|
Adults
|
%
|
|---|
|
a
|
LA513A/+
|
+/+
|
Yes
|
LA513A/+
|
1000
|
489
|
468
|
446
|
442
|
437
|
434
|
89
|
| | | | |
Wild type
| |
444
|
431
|
403
|
400
|
396
|
392
|
88
|
|
b
|
+/+
|
LA513A/+
|
Yes
|
LA513A/+
|
1000
|
442
|
420
|
404
|
399
|
393
|
383
|
87
|
| | | | |
Wild type
| |
466
|
444
|
428
|
417
|
412
|
404
|
87
|
|
c
|
LA513A/+
|
+/+
|
No
|
LA513A/+
|
540
|
274
|
265
|
235
|
208
|
155
|
7
|
2.6
|
| | | | |
Wild type
| |
233
|
225
|
214
|
212
|
209
|
206
|
88
|
|
d
|
+/+
|
LA513A/+
|
No
|
LA513A/+
|
497
|
216
|
205
|
181
|
168
|
131
|
9
|
4.2
|
| | | | |
Wild type
| |
241
|
225
|
216
|
214
|
211
|
207
|
86
|
- Adults heterozygous for LA513A were allowed to mate with wild type. a and c: transgenic males crossed to wild type females; b, d: the reciprocal cross using transgenic females. Eggs were collected and the resulting larvae reared in media supplemented with tetracycline (Tc) to 30 μg/ml (crosses a and b) or in normal, tetracycline-free media (crosses c and d). Data are the sum of at least 5 experiments. The ratio of transgenic to non-transgenic first instar larvae (L1) was approximately 1:1 (1421:1384), indicating that there was no significant differential embryonic mortality between transgenic and wild type (binomial exact test,p = 0.248). The numbers of transgenic and non-transgenic first, second, third and fourth instar larvae (L1–L4), pupae and adults resulting from these eggs is shown. In the absence of tetracycline, the transgenics showed very high (96–97%) mortality between first instar larvae and adult stages (highlighted cells); this was completely suppressed by tetracycline. Mortality of transgenics in the absence of tetracycline is strongly stage-specific, being primarily around the pupal stage (L4-pupae and pupae-adult; most affected individuals started to pupate but failed to develop beyond the earliest stages of pupal development).
