Figure 7
From: A novel role for RecA under non-stress: promotion of swarming motility in Escherichia coli K-12
RecA or RecA-GFP catalyzed ssDNA-dependent ATP hydrolysis. The RecA protein [1 μM (filled circles)] or the RecA-Htg variant [1 μM (triangles)] or increasing concentrations of RecA-GFP [0.25 (circles), 0.5 (squares), 1 (rhombuses), 1.5 (plus signs) or 2 μM (crosses)] were preincubated with circular ssDNA (10 μM) in buffer C, then 2 mM ATP was added and the reaction was incubated for a variable time at 37°C. Time zero corresponds to the moment the ATP was added.