Figure 8 From: A novel role for RecA under non-stress: promotion of swarming motility in Escherichia coli K-12 Visualization of filament formation of RecA or its variants. Circular ssDNA (10 mM in nucleotides) was pre-incubated with (1 mM) RecA (A) or variants RecA-Htg (B) or RecA-GFP (C) 20 min at 37°C in buffer C containing 20 mM NaCl and 1 mM ATPγS, and incubated for 40 min at 37°C. The specimens were prepared for electron microscopy by negative staining with 1%Back to article page