Pygo1 and Pygo2 expression in the E18.5 developing kidney. Immunofluorescence was used to determine expression patterns of the Pygo1 and Pygo2 proteins in the cortex of the E18.5 kidney. Both Pygo1 and Pygo2 (red) were localized in the nucleus, as expected. Both genes showed widespread expression, with signal detected in all components of the developing kidney, but with elevated levels in the stromal cell compartment (arrows). Epithelial cells, primarily ureteric bud in these sections, were labeled blue using E-cadherin antibody. Original magnification × 200.