Quantitative analysis of BAT-gal reporter expression in Pygo1 / Pygo2 E18.5 kidney extracts. Transgene-specific beta-galactosidase was quantified by ELISA analysis. Pygo1
+/- and Pygo1
+/+ kidneys all showed similar levels of BAT-gal expression. In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%. In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles. Each experimental group included a sample size of at least four.