cOPT reconstruction. A 10 mm fish is shown with Alcian blue and alizarin red staining the cartilage and bone respectively. The three separate reconstructions relating to the red, green, and blue channels are combined to form a single cOPT image allowing a combination of brightfield stains to be used. (A) Sagittal section. (B) Color volume rendering showing the complete developing skeleton of the 10 mm fish.