The temporal and geographic distribution of 66 STs described for N. gonorrhoeae. A neighbour-joining tree was constructed from the concatenated MLST sequences from 66 STs obtained from 149 gonococcal isolates. Each circle denotes a particular ST and the countries and regions of isolation, if known are shown. Green circles indicate STs from isolates collected in Liverpool, UK between 1981 and 1991, pink circles indicate STs from isolates collected in Liverpool, UK between 2000 and 2001 and blue circles indicate STs from isolates collected elsewhere, including geographically and epidemiologically unlinked isolates. The two closely related STs obtained from the AHU- isolates are shown.