Figure 1

Androgen-responsive intronic RNAs. (A) Hierarchical clustering of 39 intronic transcripts significantly upregulated or downregulated after androgen exposure. (B) Clusters of intronic transcripts (I-III) with similar temporal expression patterns are indicated. Average fold change within each cluster in panel B is indicated by a colored line. (C) Microarray expression pattern of 13 selected androgen-regulated intronic messages selected for orientation-specific reverse-transcription PCR (OSRT-PCR). (D) Transcriptional orientation of 13 selected androgen-regulated intronic messages relative to the correspondent protein-coding mRNA determined by OSRT-PCR. Antisense (GAS6, RAB25, ITGA6 and ADD3), sense (ACTN4, STARD3 and ERG) or simultaneous antisense and sense (DNAJC3, SAP18, KDELR2, FLJ10154, MYO5A and ATF2) transcription (right panel; antisense, sense +RT, + primer) was observed. Controls to monitor the absence of contamination with genomic DNA (right panel; control - RT, + primer) or the absence of RNA self-priming (right panel; control + RT, - primer) are indicated. Names between panels C and D refer to protein-coding RefSeq mRNAs that map to the same loci and define intronic regions where active transcription was detected.