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Table 5 The list of cuticle associated proteins, in order of expression levels, that are up-regulated in the intermoult stage (Cy3) when compared against early pre-moult crabs (Cy5)

From: Gene expression profiling of cuticular proteins across the moult cycle of the crab Portunus pelagicus

Transcript IDs (accession no.)

Gene IDs (BLAST results)

Score (bits)

E value

*M

*t

*P value

*Adjusted P value

PpCUT1 (GenBank: EF101993)

P81582 CUPC3 Cuticle protein CP1246 (CPCP1246) C. pagurus

148

3e-34

7.715

12.071

0

0.027

PpCUT2 (GenBank: EF101994)

P81582 CUPC3 Cuticle protein CP1246 (CPCP1246) C. pagurus

143

9e-33

7.397

15.388

0

0.015

PpCB1 (GenBank: EF102006)

DQ288153 C. sapidus calcified cuticle protein CP6.1

135

1e-28

7.109

14.424

0

0.018

 

ABB91678 calcified cuticle protein CP6.1 C. sapidus

83.2

3e-15

    

PpCB2 (GenBank: EF102007)

DQ288153 C. sapidus calcified cuticle protein CP6.1 mRNA

61.9

5e-06

7.089

15.538

0

0.015

 

ABB91678 calcified cuticle protein CP6.1 C. sapidus

65.5

5e-09

    

PpBD1 (GenBank: EF102013)

DQ288154 C. sapidus calcified cuticle protein CP15.0 mRNA

87.7

3e-14

6.44

14.605

0

0.017

 

P81584 CUPC5 Cuticle protein CP1876 CPCP1876 C. pagurus

145

1e-33

6.249

11.706

0

0.029

PpCUT3 (GenBank: EF101995)

P81588 CUC10 Cuticle protein CP498 (CPCP498) C. pagurus

54.3

2e-06

6.121

19.272

0

0.012

    

5.898

19.173

0

0.012

PpCB3 (GenBank: EF102008)

DQ288153 C. sapidus calcified cuticle protein CP6.1 mRNA

54.0

3e-04

5.995

13.013

0

0.022

 

ABB91678 calcified cuticle protein CP6.1 C. sapidus

79.7

4e-14

    

PpBD2 [GenBank: EF102014]

DQ288154 C. sapidus calcified cuticle protein CP15.0 mRNA

745

0.0

5.327

23.449

0

0.012

 

ABB91679 calcified cuticle protein CP15.0 C. sapidus

214

2e-54

    

PpCUT4 (GenBank: EF101996)

P81582 CUPC3 Cuticle protein CP1246 (CPCP1246) C. pagurus

42.0

3e-08

5.274

17.593

0

0.014

PpCB4 (GenBank: EF102009)

AY752734 C. sapidus arthrodial cuticle protein AMP6.0 mRNA

559

3e-156

5.176

20.083

0

0.012

 

AAV28477 arthrodial cuticle protein AMP6.0 C. sapidus

192

5e-48

4.616

20.25

0

0.012

PpCUT5 (GenBank: EF101997)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

155

3e-36

4.718

12.398

0

0.025

PpCUT6 (GenBank: EF101998)

P81587 CUPC8 Cuticle protein CP463 (CPCP463) C. pagurus

81.6

4e-14

4.26

11.666

0

0.029

PpCUT7 (GenBank: EF101999)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

80.9

7e-14

3.418

19.596

0

0.012

PpCUT8 (GenBank: EF102000)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

142

2e-32

3.351

18.96

0

0.012

PpCUT11 (GenBank: EF102003)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

41.2

0.003

3.192

15.938

0

0.015

PpCUT10 (GenBank: EF102002)

P81585 CUPC6 Cuticle protein CP434 (CPCP434) C. pagurus

76.6

3e-13

3.041

17.14

0

0.015

PpCUT9 (GenBank: EF102001)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

100

5e-20

2.963

15.21

0

0.015

PpVER1 (GenBank: EF102018)

NP_730442 LDLa domain containing chitin binding protein 1 CG8756-PA Drosophila

155

1e-38

2.585

10.905

0

0.034

  1. M = log 2 fold change in expression, t = t-statistic, and the P value t-distribution. The adjusted P values were calculated using the false discovery rate (FDR) procedure. Positive values of M and t indicate up-regulation in the Cy3 sample (* multiple values for the same transcript indicate expression levels for different cDNA probes on the microarray that make up a contig).