Gene expression profiling of cuticular proteins across the moult cycle of the crab Portunus pelagicus

Table 6 The list of cuticle associated proteins up-regulated in the late pre-moult (Cy3) stage when compared against crabs in ecdysis (Cy5)

Transcript IDs (accession no.)	Gene IDs (BLAST results)	Score (bits)	E value	*M	*t	*Adjusted P value
PpCRYP1 (GenBank: EF102021)	AF091261 Cancer magister cryptocyanin (CC1) ABB59714 cryptocyanin 2 C. magister	517	2e-143	5.334	20.137	0.01
		572	8e-162	5.07	19.923	0.01
				5.061	19.374	0.01
				4.875	16.555	0.01
				4.842	17.141	0.01
				4.747	15.569	0.01
				4.682	13.94	0.01
				4.536	17.265	0.01
				4.502	12.822	0.01
				4.453	12.362	0.01
				4.413	12.648	0.01
				4.39	12.755	0.01
				4.374	15.933	0.01
				4.354	10.194	0.015
				4.311	13.787	0.01
				4.179	10.362	0.015
				3.937	8.784	0.022
PpCRYP2 (GenBank: EF102022)	DQ230982 C. magister cryptocyanin 2 (Cc2) AAD09762 cryptocyanin C. magister	157	7e-35	4.873	16.433	0.01
		226	8e-68	4.304	11.836	0.011

M = log ₂ fold change in expression, t = t-statistic, and the P value t-distribution. The adjusted P values were calculated using the false discovery rate (FDR) procedure. Positive values of M and t indicate up-regulation in the Cy3 sample (* multiple values for the same transcript indicate expression levels for different cDNA probes on the microarray that make up a contig).

ISSN: 1741-7007