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Table 1 15N-labelling of free amino acids in the hemolymph of ants fed with [15N] urea

From: Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia

  Calculated natural abundance Urea feeding experiments (control) [15N]Urea feeding experiments (labelling)
  M+1 abundance (fraction %) M+1 abundance (fraction %) [15N]AA (mol %) M+1 abundance (fraction %) [15 N]AA (mol %)
Gly 7.6 6.8 ± 0.3 -0.8 ± 0.3 11.7 ± 0.8 4.2 ± 0.8
Ala 8.5 7.8 ± 0.6 -0.7 ± 0.6 16.7 ± 1.8 8.2 ± 1.9
Glu 11.1 10.4 ± 0.3 -0.8 ± 0.3 20.1 ± 2.5 8.9 ± 2.5
Asp 10.3 9.6 ± 0.2 -0.7 ± 0.2 16.1 ± 1.7 5.8 ± 1.7
Met 10.4 10.8 ± 1.1 0.4 ± 1.1 13.4 ± 0.9 3.0 ± 0.9
Phe 12.5 11.8 ± 0.3 -1.7 ± 0.3 16.4 ± 1.2 2.9 ± 1.2
  1. After feeding experiments, free amino acids (AA) from hemolymph of 4–6 ants were derivatized and analyzed by GC-MS. The abundance of the first isotope peak, i.e. the amount of naturally occurring AA containing a heavy isotope (either 13C or 15N; M + 1) was determined from the mass spectra of amino acids, and additional 15N-incorporation (mol %) was calculated. Both non-essential and essential (bold) AA were found to be labelled. Values are obtained from at least three independent experiments and relative amount averaged over samples (mean ± SE).