Dynacortin distribution during cAMP stimulation. A. GFP-dynacortin localization in wild-type cells was visualized by fluorescence microscopy. The white dot denotes the position of the micropipette. B. Linescan of dynacortin signal intensity of the cell in A marked with a white line. The linescan begins at the front (towards the micropipette) of the cell. C. TIRF imaging of wt: GFP-dyn cells shows dynacortin enrichment in the front half of the cell. The white dot denotes the position of the pipette. D. Linescan of cell in C with the white line. E. High magnification TIRF image of a wt: GFP-dyn cell. The arrow identifies the direction of the micropipette. Dynacortin is localized to cell surface structures that are enriched at the front half of the cell. Scale bars, 10 μm.