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Figure 1 | BMC Biology

Figure 1

From: Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling

Figure 1

O -fucose is not required during embryonic neurogenesis. Embryos immunostained with anti-ELAV (green) and either anti-HRP or anti-OFUT1 (magenta) antibodies, as indicated. Panels marked prime show separate channels of the same embryo. (A)Wild-type embryo. (B) Ofut1 4R6 mutant embryo from Ofut1 4R6 germline clone. A neurogenic phenotype is revealed by the expansion of ELAV staining. (C) Ofut1 4R6 mutant embryo from Ofut1 4R6 germline clone expressing Ofut1 R245Afrom a genomic rescue construct(Ofut1 R245A [18.1]). OFUT1R245A expression is visible. ELAV staining shows the absence of a neurogenic phenotype. (D) Gmd 1 mutant embryo derived from Gmd 1 germline clone. Absence of fucosylation was confirmed by the absence of the HRP epitope; neurogenesis appears normal.

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