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Figure 6 | BMC Biology

Figure 6

From: Hue-shifted monomeric variants of Clavulariacyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

Figure 6

Live cell imaging of mTFP1 fusion vectors. (A)-(D) Laser scanning confocal images of a single HeLa cell expressing mTFP1-H2B-6 (N-terminus; human) progressing through prophase, metaphase, anaphase and interphase, respectively. (E)-(H) Spinning disk confocal images selected from a time-lapse series of HeLa cells expressing mTFP1-annexin (A4)-12 (C-terminus; human) during ionomycin-induced translocation to the plasma and nuclear membranes [35]: (E) 0 min, ionomycin added; (F) 5 min; (G) 7 min; (H) 9 min. Scale bars represent 10 μm.

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