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Figure 3 | BMC Biology

Figure 3

From: Characterization of the proneural gene regulatory network during mouse telencephalon development

Figure 3

Expression analysis of predicted Ngn2 and Mash1 targets through in situ hybridization of overexpressing and mutant embryos. (a) Expression of the predicted Ngn2 targets Nhlh1, zfp238, Rbdh4, Mfng, and Elavl4 (the latter two are predicted common targets of Ngn2 and Mash1) in E10.5 embryos electroporated with a Ngn2 expression vector and cultivated for one day (left panels), in E13.5 wild-type embryos (middle panels), and in E13.5 Ngn2 mutant embryos (right panels). (b) Expression of the predicted Mash1 targets Gadd45g, Pdpn, Mfng, and Elavl4 in E10.5 embryos electroporated with a Mash1 expression vector and cultivated for one day (left panels), in E12.5 wild-type embryos (middle panels), and in E12.5 Mash1 mutant embryos (right panels). A * in the left panels indicates cells overexpressing the target genes. Arrowheads in the right panels indicate cells having downregulated the target genes.

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