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Figure 1 | BMC Biology

Figure 1

From: The brown adipocyte differentiation pathway in birds: An evolutionary road not taken

Figure 1

Appearance of cells with mammalian brown fat-like phenotype in embryonic chicken mesenchyme in vitro. (a) Oil-red and hematoxylin staining of light micrographs of limb bud mesenchyme (LBM) cells grown in adipocyte differentiation medium (ADM) for 22 days with 10% horse serum. (b) Transmission electron micrograph of a single cell from 22-day culture as in (a); L, lipid droplet. (c)-(f) Oil-red and hematoxylin staining of light micrographs of 8-day cell cultures.(c) Adipocytes from LBM cells grown in ADM with 10% horse serum. (d) Adipocytes from vascular stromal cells (VSCs) grown in ADM with 10% horse serum. (e) Adipocytes from LBM grown in ADM with 10% chicken serum. (f) LBM cells grown in ADM with 10% FBS. Scale bars: (a) 10 μm, (b) 1 μm; (c)-(f) 50 μm

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