Figure 3

Avian brown adipocyte-like cells express common adipocyte markers and specific brown adipocyte markers. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of relative expression of genes common to differentiated phenotype of white and brown adipocytes and specific to brown fat differentiation in limb bud mesenchyme (LBM), limb bud preadipocytes (LBPAs), avian brown adipocyte-like cells (ABALCs) in 8-day cell culture, vascular stromal cells adipocytes (VSCAs) and white adipocytes (WAs) from abdominal fat of 20-day chicken embryos. (a) Fatty acid binding protein, FABP4. (b) Peroxisome proliferator activated receptor γ, PPARγ. (c) Peroxisome proliferator-activated receptor γ coactivator 1α, PGC-1α. (d) Type II iodothyronine deiodinase, DiO2 and peroxisome proliferator activated receptor α, PPARα. Each treatment was repeated three times. Measurements were in triplicate and normalized to β-actin mRNA levels. Relative mRNA levels were analyzed by one-way analysis of variance and means were compared using the Tukey test. Results are shown as means ± standard error of the mean.