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Table 1 Assembly of TMD and TMD:FP complex of HA as probed by the Rhodamine self-quenching. The FP peptide of HIV gp41 was used as a negative control. Values are expressed as a percentage of the Rhodamine intensity in the presence of 0.2% Triton X-100.

From: Membrane interaction and structure of the transmembrane domain of influenza hemagglutinin and its fusion peptide complex

A

B

pH

A

A+B

Difference

Rho-TMD (HA)

FP (HA)

5.0

8.7

9.3

+0.6

  

7.4

12.9

13.0

+0.1

Rho-TMD (HA)

FP (HIV)

5.0

10.4

11.3

+0.9

  

7.4

12.1

13.0

+0.9

Rho-FP (HA)

TMD (HA)

5.0

35.5

55.9

+20.4

  

7.4

63.1

70.4

+7.3

Rho-FP (HIV)

TMD (HA)

5.0

63.2

63.1

-0.1

  

7.4

79.5

77.9

-0.4