Table 2 The secondary structure and orientation of helix, beta sheet and lipid acyl chain of FP, TMD and FP/TMD 1:1 complex in DMPC:DMPG 1:1 vesicular solution with L/P = 50 at pH 5.0. Values were obtained by averaging three or four sets of data.
| Lipid | FP | TMD | Complex | |
|---|---|---|---|---|
| Secondary structure | ||||
| α-helix (%) | 26 ± 3 | 64 ± 1 | 46 ± 7 | |
| β-sheet (%) | 55 ± 5 | 20 ± 2 | 28 ± 1 | |
| Unordered (%) | 9 ± 3 | --- | 9 ± 8 | |
| β-turns (%) | 11 ± 3 | 17 ± 4 | 17 ± 3 | |
| Helix axis orientation | ||||
| R ATR | 1.82 ± 0.01 | 2.46 ± 0.22 | 2.07 ± 0.08 | |
| Θ (°) | 60 ± 1 | 34 ± 1 | 49 ± 2 | |
| Beta-strand orientation | ||||
| R ATR | 1.18 ± 0.10 | N.A. | 1.28 ± 0.20 | |
| Φ (°) | 56 ± 5 | N.A. | 52 ± 7 | |
| Acyl chain tilt angle | ||||
| R ATR | 1.09 ± 0.09 | 1.78 ± 0.45 | 1.31 ± 0.04 | 1.62 ± 0.12 |
| δ (°) | 27 ± 4 | 49 ± 12 | 36 ± 1 | 45 ± 4 |