Transcript profiles for copper exposed worms, 329 transcripts significantly differently expressed as a result of copper treatment (ANOVA). (A) Hierarchical cluster analysis and heat map, individual replicates. Colours of heat map indicate up-regulation (red) and down-regulation (green) of transcripts. (B) hierarchical cluster analysis and heat map, dose group averages. (C) PCA, first two components. (D)-(G) relative expression profiles (transcripts significantly different at the p = 0.05 level) fall into four clusters (K-means). Colours of different groups for (D)-(G) are for visualisation only.