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Figure 6 | BMC Biology

Figure 6

From: A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)3 and Sb(OH)3across membranes

Figure 6

Detection by Western blotting of AtNIP6;1, Atnip6;1Δ 2–29 and Atnip6;1Δ 2–69 expressed in yeast. We ran 20 μg of microsomal membrane proteins from (a) Δfps1 and (b) Δfps1 Δacr3 Δycf1 transformed with the empty vector or AtNIP6;1, Atnip6;1Δ2–29 or Atnip6;1Δ2–69 on 12% SDS polyacrylamid gels. Proteins were (A) stained with commassie brilliant blue or (B) transferred to nitrocellulose membrane for probing with an anti-AtNIP6;1 specific antibody. M, PageRuler™ Prestained Protein Ladder (Fermentas). The arrows point towards bands that probably represent monomeric forms of Atnip6;1Δ2–29 and Atnip6;1Δ2–69. Arrowheads point towards bands that probably represent oligomeric forms of the proteins.

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