Comparative analyses of 3' splice sites, GC contents, and cross-species sequence conservation. (A) The conservation of consensus 3'-end splice sites (TTTTCAG) of confirmed transcripts and transcripts with exon-intron boundary 454 expressed sequence tag hits. (B) A comparison of 454 expressed sequence tags and Caenorhabditis elegans whole genome for guanine-cytosine content of different genomic regions. (C) Average ClustalW alignment score comparisons for intergenic regions with or without 454 expressed sequence tags for different numbers of orthologous sequences. (D) Chart showing 3524 unique Caenorhabditis elegans intergenic 454 expressed sequence tags mapped to C. briggsae, C. remanei, and C. brenneri.