Table 2 PCR primers used for amplification of the α- and β-like globin genes including GBY from the platypus gDNA and cDNA
Gene | Forward Primer | Reverse Primer | gDNA (bp) | cDNA (bp) |
|---|---|---|---|---|
ζ | GGCCGACAAGACCGCAGTCATCTCCC | CCCGATGGCGCTGATGACT | 527 | 190 |
ζ 1 | TGACCAAAGGCGACAAGACCT | CCCCGATGGCACCGATGACC | 534 | 198 |
α D | GAGGCTGTGAAGAACCTGGA | GGTGTACTCCCCTTGCAGAT | 1793 | 153 |
α 2 | TGGCCCACCTCGATGACCTGG | GGGAAGGTGTCTGGCCACC | 289 | 134 |
α 1/α 3 | GCAAGGCCGCCGGTCACGGC | CGCTGTCCATGTCATCGAAGTGCC | 597 | 192 |
ω | ATTGTGTCCATCTGGGGAAA | GCTTGGCAAAGTTGCTCTTC | 488 | 232 |
GBY | CTGGAAACAGGTGTGCAAGA | CTATCTCCGGGGTGTAGCAG | 3202 | 149 |
ε | ATCTGAGCGCTGAGGAGAAG | GACAGGTTGCCGAAGGAGTCA | 285 | 142 |
β | CTGTGGGGGAAAGTGAACAT | GGTCAGCACCTTAGCACCAT | 321 | 168 |