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Figure 3 | BMC Biology

Figure 3

From: Erythropoietin enhances hippocampal long-term potentiation and memory

Figure 3

Neurophysiology of acute hippocampal slices: Extracellular recordings. (a-b) Input-output relation is not altered at Schaffer collateral-CA1 synapses in EPO-treated mice. (a) Sample recordings at 50% of maximal response (average of four traces) are shown for control and EPO-treated mice. (b) Input-output curve as a measure of baseline excitatory synaptic transmission: fEPSP slope, plotted against the stimulation strength, is not altered in EPO-treated mice compared to control (P = 0.3094). Inset: Half maximal stimulation strengths are not significantly different. (c-d) Paired-pulse facilitation is enhanced in EPO-treated mice. (c) Sample traces are presented. (d) Paired-pulse ratio (fEPSP slope for the second stimulus/fEPSP slope for the first stimulus) at inter-stimulus intervals of 25–150 ms is significantly greater in EPO-treated mice. (e-h) Increased LTP at Schaffer collateral CA1 synapses in EPO-treated mice. (e) Sample traces of responses are shown before and after high frequency stimulation (HFS; 3 × 100 Hz for 1 s each, 20 s interval). (f) Long-term potentiation elicited by HFS: Slopes of fEPSP are normalized to baseline and plotted against time. Time-point 0 represents application of HFS. (g) Magnitude of STP, determined as maximal responses within 1 min after HFS, is significantly greater in EPO-treated mice. (h) Magnitude of LTP, determined as responses between 50 and 60 min after HFS, is significantly greater in EPO-treated mice. (i-l) Increased STD at Schaffer collateral-CA1 synapses in EPO-treated mice. (i) Sample traces of responses are shown before and after low frequency stimulation (LFS; 1 Hz for 900 stimulations). (j) Long-term depression elicited by LFS: Slopes of fEPSP are normalized to baseline and plotted against time. Time 0 represents application of LFS. (k) Magnitude of STD, determined as maximal responses within 1 min after LFS, is significantly greater in EPO-treated mice. (l) Magnitude of LTD, determined as responses between 50 and 60 min after LFS, is not significantly changed in EPO-treated mice (P = 0.0869).

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