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Table 2 Effects of PUFA on the distribution of cholesterol in ScGT1 cells

From: Docosahexaenoic and eicosapentaenoic acids increase prion formation in neuronal cells

Treatment Cholesterol ng/ml
  Total Free Esterified
None 590 ± 54 515 ± 29 75 ± 26
DHA (1 μM) 544 ± 39 411 ± 26 # 133 ± 31 @
EPA (1 μM) 553 ± 28 438 ± 25 # 115 ± 20 @
LNA (1 μM) 607 ± 26 548 ± 43 59 ± 18
LA (1 μM) 572 ± 74 495 ± 52 77 ± 28
Simvastatin (5 μM) 438 ± 46 * 354 ± 41 # 84 ± 32
Cholesterol myristate (10 μM) 762 ± 55 * 538 ± 51 224 ± 41 @
Cholesterol arachidonate (10 μM) 734 ± 47 * 545 ± 38 189 ± 32 @
  1. The amounts of total, free and esterified cholesterol in extracts from ScGT1 cells treated for 24 hours with PUFA, simvastatin, or with cholesterol esters as shown. Amounts of total, free and esterified cholesterol were expressed as the mean average (ng/ml) ± SD, n = 9. Amounts of total cholesterol (*), free cholesterol (#) or esterified cholesterol (@) significantly different from that of untreated cells (P < 0.01).