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Table 3 Treatment with PUFA does not affect the amounts of PrPC in neuronal membranes

From: Docosahexaenoic and eicosapentaenoic acids increase prion formation in neuronal cells

Treatment PrPC (ng/ml)
  Whole cell extract Lipid raft fraction Bulk fraction
None 31.6 ± 4.8 25.5 ± 2 2.9 ± 0.9
1 μM AA 30.2 ± 3.1 26.8 ± 2.9 2.8 ± 0.5
1 μM DHA 33.2 ± 4.4 26.6 ± 3.4 3.8 ± 0.8
1 μM EPA 34.5 ± 2.9 27.8 ± 3.1 3.1 ± 0.3
1 μM LA 29.9 ± 4.1 25.9 ± 2.8 3.4 ± 0.8
1 μM LNA 28.8 ± 4.7 24.5 ± 2 3.5 ± 1.2
2 μM Simvastatin 35.6 ± 4.2 7.2 ± 2.2 * 25.9 ± 3.8 *
  1. The amounts of PrPC in whole cell extracts, detergent insoluble membranes (lipid raft fraction) and the detergent soluble membrane (bulk fraction) from primary cortical neurons treated with 1 μM PUFA or 2 μM simvastatin for 24 hours. Amounts of PrPC were measured by ELISA and are expressed as the mean average PrPC (ng/ml) ± SD, n = 12. * = amounts of PrPC significantly different from that of untreated cells (P < 0.01).