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Table 4 Treatment with DHA or EPA increases the amounts of PrPC at the cell surface

From: Docosahexaenoic and eicosapentaenoic acids increase prion formation in neuronal cells

Treatment Cell surface PrPC (ng/106 cells)
  Cortical Neurons GT1 cells ScGT1 cells
None 2.9 ± 0.4 1.8 ± 0.6 4.1 ± 0.8
1 μM AA 3.1 ± 0.4 2.1 ± 0.4 3.7 ± 1.1
1 μM DHA 4.8 ± 0.4 # 3.1 ± 0.6 # 8.6 ± 1.6 #
1 μM EPA 4.5 ± 0.5 # 2.8 ± 0.5 # 6.7 ± 1.2 #
1 μM LA 3.4 ± 0.6 2.5 ± 0.6 5 ± 0.7
1 μM LNA 3.2 ± 0.6 2.3 ± 0.5 5.8 ± 0.8 #
2 μM Simvastatin 0.6 ± 0.3 * 0.5 ± 0.2 * 0.7 ± 0.4 *
  1. The amounts of PrPC at the surface of 106 cells treated with 1 μM PUFA or 2 μM simvastatin for 24 hours were measured following digestion with PI-PLC (0.2 units/ml). Amounts of PrPC were measured by ELISA and are expressed as the mean average PrPC (ng/106 cells) ± SD, n = 12. # = amounts of PrPC significantly higher than that of untreated cells (P < 0.01). * = amounts of PrPC significantly lower than that of untreated cells (P < 0.01).