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Table 4 Treatment with DHA or EPA increases the amounts of PrPC at the cell surface

From: Docosahexaenoic and eicosapentaenoic acids increase prion formation in neuronal cells

Treatment

Cell surface PrPC (ng/106 cells)

 

Cortical Neurons

GT1 cells

ScGT1 cells

None

2.9 ± 0.4

1.8 ± 0.6

4.1 ± 0.8

1 μM AA

3.1 ± 0.4

2.1 ± 0.4

3.7 ± 1.1

1 μM DHA

4.8 ± 0.4 #

3.1 ± 0.6 #

8.6 ± 1.6 #

1 μM EPA

4.5 ± 0.5 #

2.8 ± 0.5 #

6.7 ± 1.2 #

1 μM LA

3.4 ± 0.6

2.5 ± 0.6

5 ± 0.7

1 μM LNA

3.2 ± 0.6

2.3 ± 0.5

5.8 ± 0.8 #

2 μM Simvastatin

0.6 ± 0.3 *

0.5 ± 0.2 *

0.7 ± 0.4 *

  1. The amounts of PrPC at the surface of 106 cells treated with 1 μM PUFA or 2 μM simvastatin for 24 hours were measured following digestion with PI-PLC (0.2 units/ml). Amounts of PrPC were measured by ELISA and are expressed as the mean average PrPC (ng/106 cells) ± SD, n = 12. # = amounts of PrPC significantly higher than that of untreated cells (P < 0.01). * = amounts of PrPC significantly lower than that of untreated cells (P < 0.01).