Testing the Tom-2A-GFP construct in chick embryos. (A-C) Three-dimensional volume rendering of a confocal image stack of embryonic chick neural crest cells electroporated with the Tom-2A-GFP construct under the control of a CMV enhancer. The enhanced green fluorescent protein (EGFP) and TdTomato segregate to the nucleus and plasma membrane respectively, indicating that the 2A peptide functions in chick cells. (A) EGFP fluorescence. (B) TdTomato fluorescence. (C) Merge of EGFP (green) and TdTomato (magenta) fluorescence. Scale indicators are rendered in perspective along with the confocal image data and do not translate reliably to a two-dimensional image, therefore, no scale bar is depicted. As an approximate indicator, the two cells at the right of the image that have just divided are, together, between 15 and 20 μm long.