Function of 2A peptide in E8.5 embryos. (A-A") Transverse section through an E8.5 transgenic embryo. (B-B") Transverse section through the neural tube of an E8.5 transgenic embryo. Note the 'ring' of dividing cells lining the inside of the neural tube. (C-D") High magnification view of neural tube cells expressing Tom-2A-GFP, stained with 4',6-diamidino-2-phenylindole (DAPI) (nucleus) and phalloidin (sub-cortical actin marking plasma membrane), confirming that enhanced green fluorescent protein (EGFP) and TdTomato are expressed in mutually exclusive compartments, in the nucleus and plasma membrane respectively. (A, B, C) EGFP fluorescence. (A', B', C') TdTomato fluorescence. (A", B", C") Merge of EGFP (green) and TdTomato (magenta) fluorescence. (D) DAPI fluorescence. (D') Atto647N-phalloidin fluorescence. (D") Merge of DAPI (green) and Atto647N (magenta). The scale bar in (A) represents 63 μm in (A-A"), 31 μm in (B-B") and 10 μm in (C-D").