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Table 2 Immune-related ESTs and comparison of gene expression between naive larvae, mock-infected larvae and larvae challenged with Escherichia coli.

From: Identification of the Weevil immune genes and their expression in the bacteriome tissue

EST Protein description E-value Target Organism UniProt Acc. Num. qRT-PCR Fold change
  Antibacterial peptides     sterile injury septic injury
INF-18 Coleoptericin 3E-15 Zophobas atratus P80032 11.4* 86.1* (*)
INF-42 Diptericin A 2.6 Glossina morsitans Q8WTD5 > 10* > 300* (*)
INF-145 Acaloleptin A 2E-15 Acalolepta luxuriosa Q76K70 1.8 43.1*
INF-163 Cecropin A1 0.68 Drosophila mauritiana P81685 1 31.5*
INF-165 Sarcotoxin II-1 0.67 Sarcophaga peregrina P24491 0.8 31.6*
INF-217 Tenecin-1 3E-13 Tenebrio molitor Q27023 9.7* 314.9* (*)
INF-479 Luxuriosin 0.18 Acalolepta luxuriosa Q60FC9 2.8* 4.5*
  Lysozymes      
INF-152 Lysozyme i-1 1E-05 Anopheles gambiae Q6GU90 7.8* 5.2*
INF-282 Lysozyme C-1 6E-17 Anas platyrhynchos P00705 7.1* 5.4*
  PGRP      
INF-9 PGRP sb2 7E-57 Aedes aegypti Q1HRH3 2.3* 6.7* (*)
INF-441 PGRP 9E-38 Biomphalaria glabrata A0T2Q1 7.8* 11.6*
  Immune regulator      
INF-359 TOLLIP 3E-48 Mus musculus Q9QZ06 1.2 1.7
  Phenoloxidase pathway      
INF-506 PPAF 2E-15 Holotrichia diomphalia Q9GRW0 2.8* 2.7*
INF-74 Serpin-4A 2E-20 Manduca sexta Q6Q2D8 2.8* 2.9*
  Proteases a      
INF-20 IMPI 2E-11 Galleria mellonella P82176 2.3* 3.4*
INF-91 Cysteine-rich venom-like protein 7E-09 Aedes albopictus Q5MIW2 3.4* 7.5* (*)
INF-258 Pattern recognition serine proteinase 7E-28 Manduca sexta Q69BL0 ND ND
INF-459 Hemolymph proteinase 17 3E-10 Manduca sexta Q5MPB8 1.7 4.4
INF-515 Trypsin-like serine proteinase 5E-27 Anthonomus grandis Q64ID5 1.4 1.1
  Cytoskeleton      
INF-13 profilin 3E-29 Apis mellifera Q6QEJ7 0.9 0.8
- actin - Sitophilus zeamais - 1 1.3
  1. ESTs were analyzed as described in Materials and Methods and classified according to gene family or function. Transcripts were quantitated by qRT-PCR in untreated aposymbiotic larvae (control), in larvae six hours after a mock-infection (sterile injury) or an E. coli infection (septic injury). The fold-change of gene expression after sterile or septic injury is expressed relative to the transcript levels in untreated larvae. For each sample, the transcript level is estimated from the mean of three independent measurements after normalization with the expression of the gapdh gene. The transcript amounts of the genes corresponding to the EST INF-42 and INF-258 are too weak to be quantified in the control larvae and in all the samples, respectively. Comparisons of transcript levels between the three samples were made using nonparametric tests. A significant increase in transcript level after sterile or septic injury is indicated by an asterisk (p < 0.05). A significant difference between sterile and septic injury is indicated by a second asterisk (p < 0.05). Acc. Num., accession number; ND, non-determined. a. Proteases can represent either signaling proteins or immune effectors.
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