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Figure 8 | BMC Biology

Figure 8

From: The molecular basis of defective lens development in the Iberian mole

Figure 8

Lens fibre cells proliferation in the Iberian mole. (A) 3,3'-Diaminobenzidine tetrahydrochloride-immunostaining for PH3 on both mouse and mole wax sections at different embryonic stages. During mouse lens differentiation, proliferating cells (immunoreactive for PH3) are always found in the anterior epithelium (E12, E15). In the mole, very few PH3-positive cells were detected between the s4c and s5c stages, and most of them were located in the posterior region of the lens (arrows). No proliferation was observed in the lens at s7 and subsequent stages. (B) Double-immunofluorescence for PH3 (red) and FOXE3 (green) on wax sections of s5b and s5c mole samples. All the PH3-positive cells showed immunoreactivity against the anti-FOXE3 antibody. The nuclei are stained blue with 4'6-diamidino-2-phenylindole stain. Photomicrographs were taken using a single bandpass fluorescence mirror unit and merged with 'The Gimp' software. Black scale bar represents 200 μm in all figures of A, and white scale bar represents 50 μm in all figures of B.

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